فهرست نشریات علمی دسترسی آزاد ایران

In the present study, ZnONPs were synthesized using Tannic acid. During phytosynthesis of ZnONPs, the color of the solution changed from colorless to red color. Our analysis of UV test, found that maximum absorption is at 268 nm. The obtained four energy band gaps of 0.85, 1.25, 2.45 and 3 eV Indicate the presence of two quantitative wells. The functional groups present on the surface of TA and ZnONPs were investigated by FTIR analysis, the results found that there were more than five bands in synthesized ZnONPs were shifted towards higher or lower than that found in the standard pure of Tannic acid which is good evidence for the success of the reaction between tannic acid and ZnONPs. The result of XRD analysis found hexagonal structure nanoparticles which have 27.2 nm calculating by Sherrer equation. The size which measured by AFM was 38.18 nm, root mean square (Sq) was 18.28 nm, maximum height 162.4 nm, Arithmetic mean height 3.84 nm. SEM image of ZnONPs found rod shape particles. The prepared zinc oxide nanoparticles were assessed for their toxicological effect on the human skin squamous carcinoma cell line (HSSC) in pass 27 and human oral squamous carcinoma cell line (HOSC) in pass 22. For HSSC cell line, the IC50 was 200, 200, and 150 µg/mL for the periods of incubation of 24, 48, and 72 hours, respectively. For HOSC cell line, the IC50 was 225, 182, and 167 µg/mL for the periods of incubation of 24, 48, and 72 hours, respectively. It is evident from the results that the higher concentration of ZnONPs found to inhibit mitotic index, blastogenic index and caused chromosomal aberrations in blood lymphocytes.