Preparation of Co3O4 Nanoparticles and Studying the Antibacterial Activity on Bacteria Isolated from the Aquatic Environment of the Diyala River

نویسندگان

1 Department of Biology. College of Education for Pure Science, University of Diyala , Ba’agubah, Diyala, Iraq

doi
10.22052/JNS.2024.04.005
چکیده

The sol-gel method, which involved dissolving cobalt nitrate in distilled water to create a solution, adding urea, heating the solution for an hour at 110 °C to create a gel, and drying the gel for an additional hour at 280 °C to create cobalt oxide powder, was used in this study to create cobalt oxide nanoparticles. The finished product was compared to the (ICCD) card number 96-900-5889 and analyzed using the X-ray diffraction method. It was found that the material has a cubic crystal structure. The crystal size (29.2 nm) was calculated using the Debye-Scherer equation. The antibacterial activity of cobalt oxide nanoparticles was then examined on a culture of Escherichia coli bacteria cultured in Molar Hinton agar by producing cobalt oxide as a circular disc and placing it inside the bacteria. It was discovered that a zone had developed around the disc, suggesting that no bacteria had grown there. It suggests that certain microbes are susceptible to the antibacterial effects of cobalt oxide nanoparticles.