فهرست نشریات علمی دسترسی آزاد ایران

Urinary tract infections (UTIs) are one of the most common infectious diseases. Silver nanoparticles (AgNPs) are widely used due to their antimicrobial and antibiofilm activities. In the current study, 75 urine samples were collected from patients with urinary tract infection (UTI), with their ages ranging from (10-85) years, who visited Al-Musayyib general hospital and Alexandria general hospital/Babylon province during the period from 2nd January to 14th February 2024. All the urine samples were subjected to standard bacteriological processes and characterized based on their culture morphology and microscopic examination, as well as the biochemical tests. AgNPs were prepared by chemical methods and characterized via fourier transform infrared spectroscopy (FTIR), Zeta potential (ZP) , scanning electron microscope (SEM) and transmission electron microscope (TEM). Well-diffusion was used to screen the antimicrobial effects of AgNPs on the six isolated bacteria Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus and Enterococcus faecalis. Minimal-bactericidal concentrations (MBC) of AgNPs were determined to study their antibiofilm effect at minimum-inhibitory concentrations (MIC). The highest urinary tract infection rate was shown to be with Klebsiella pneumonia (25.33%) followed by E. coli (22.67%), while the lowest infection rate was with Enterococcus faecalis (6.67%). and result Showed that all isolates were strong biofilm producers. Exposure of the isolates to the AgNPs resulted in pronounced inhibition zones and reduced biofilms at (MIC) values.These results indicate that AgNPs with an aptitude to disrupt biofilm development for pathogenic bacteria strains it caused  UTI.