Biocompatibility and Toxicity Assessment of Gold Nanoparticles in NMRI Mice
نویسندگان
1 Samarkand State University named after Sharof Rashidov, University Boulevard, 15, Samarkand, 703004, Uzbekistan
2 Education Quality Control Department, Tashkent State Technical University, Tashkent, Republic of Uzbekistan
3 Scientific Research Department, Tashkent State Technical University, Tashkent, Republic of Uzbekistan
4 Tashkent State Technical University, Tashkent, Republic of Uzbekistan
5 Department Clinic Pharmacology, Bukhara State Medical University, Uzbekistan
6 Department of Fruits and Vegetables, Urganch State University, Uzbekistan
7 Department of rehabilitation, Tashkent Medical Academy, Uzbekistan
8 Department of Obstetrics and Gynecology, Urgench branch of Tashkent Medical Academy, Uzbekistan
9 Department of Irrigation and Melioration, Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan & Western Caspian University, Scientific researcher, Baku, Azerbaijan
10 Faculty of Geology and Mining Metallurgy, Tashkent State Technical University, Tashkent, Republic of Uzbekistan
11 Department of Propaedeutics of Pediatrics of Pediatric Faculty of the Samarkand State Medical University, Uzbekistan
12 Primary Education Department, Termez State University, Uzbekistan
13 Termez State University, Termez, Uzbekistan
doi
10.22052/JNS.2024.03.0O9چکیده
In the realm of biomedical applications, substantial interest has been drawn to gold nanoparticles (AuNPs) because of their distinctive characteristics. However, their biocompatibility and potential toxicity remain a concern. This study aims to evaluate the biocompatibility and toxicity of AuNPs in NMRI mice. The synthesis and characterization of AuNPs in three distinct sizes - 10, 20, and 50 nm - were accomplished through the dual application of DLS (dynamic light scattering), which measures hydrodynamic size, and TEM (transmission electron microscopy), which reveals particle morphology, allows for a multifaceted investigation of nanomaterials. NMRI mice were randomly divided into four groups (n=10 per group): control, AuNP-10, AuNP-20, and AuNP-50. A one-time intravenous injection of either AuNPs (at a concentration of 1 mg per kg of body mass) or a saline solution (serving as the control) was given to the experimental mice. Body weight, food intake, and clinical signs were monitored daily for 14 days. For the purpose of conducting hematological and biochemical examinations, specimens of blood were obtained from the subjects. Histopathological examinations of major organs were performed. AuNPs were successfully synthesized and characterized, showing uniform size distribution and stability. No significant differences in body weight, food intake, or clinical signs were observed among the groups. Hematological and biochemical parameters remained within normal ranges, with no significant alterations. Histopathological examinations revealed no abnormalities or signs of toxicity in the major organs. The AuNPs demonstrated excellent biocompatibility and did not induce any significant toxicity in NMRI mice at the administered dose.
