Biosynthesis of Silver Nanoparticles for Combating Foodborne Fungi Penicillium Digitatum and Aspergillus Flavus

نویسندگان

1 Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq

2 Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq

3 Department of Biology, College of Science, Al-Muthanna University, Al-Muthanna, Iraq

doi
10.22052/JNS.2024.03.025
چکیده

Silver nanoparticles biosynthesized from the plant Quercus infector (Q. infector) were tested to evaluate its antifungal potential as a growth inhibitor against some fungi species named Penicillium digitatum (P. digtatium) and Aspergillus flavus (A. flavus) isolated from foodstuffs in the local market of Al-Najaf Province/ Iraq. The results stated that the highest inhibition rate was observed for P. digitatum at 3 mM concentration (90.97±1.72%), while the inhibition percentages for 1 mM (83.29±1.05%) and 2 mM (85.44±1.23%), indicating a concentration-dependent trend. In regards to A. flavus, the findings showed that the highest inhibition percentages were observed at 3 mM concentration (89.36±0.57%), while for 1 mM (73.7±1.69%) and 2 mM (85.33±0.91%), showing a similar trend. The mean percentage inhibition across all fungi was highest at 1 mM (84.24%) compared to 2 mM (79.90%) and 3 mM (75.79%). These results emphasize the antifungal efficacy which increased with the concentration of silver nanoparticles, with P. digitatum showing higher sensitivity compared to A. flavus. The results also indicate that fungal species treated without nanoparticles showed significantly lower inhibition rates (76.29% and 77.77% for Penicillium sp. and Aspergillus sp., respectively, without nanosilver treatment). The silver nanoparticles were characterized using UV-Vis spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR).