فهرست نشریات علمی دسترسی آزاد ایران

The present study aimed to isolate the bacterial strain Streptomyces fradiae from soil and screen it for its ability to biosynthesize PtNPs and characterize and evaluate the antibacterial potential of the manufactured PtNPs. Streptomyces fradiae was isolated from soil samples using the serial dilution method. The isolated bacteria were identified using a light microscope, then diagnosed by biochemical examinations, and lastly by the Polymerase chain reaction (PCR) for molecular diagnostics. The results confirmed the presence of Streptomyces fradiae depending on the amplification of 16s RNA fragments. That was followed by the bacterial screening to examine their ability for use in the production of platinum nanoparticles. The formation of biogenic platinum nanoparticles was confirmed by physical characterization for example UV-VIS spectrophotometer exposed the presence of UV spectrophotometer peak in wavelengths at 362 nm. The results of (TEM) in the electron transmission microscope exposed that the biogenic platinum NPs were in sizes between (2.44-29.57) nm. The field emission scanning microscope also exhibited the examination of surface morphology, shape, and size, where the average diameter for PtNPs was calculated and the final value for this diameter was 5.55 nm. The Zeta potential analyses showed that platinum nanoparticles gained negative surface charges at -28.6 mV. The antibacterial activity of the platinum nanoparticles was evaluated against pathogenic bacteria, there were five species isolated from the hospital, and they exhibited an inhibitory effect on the tested bacterial isolates.