فهرست نشریات علمی دسترسی آزاد ایران

During the current investigation in the field of clinical research, efforts were made to synthesize gold oxide nanoparticles (Au2O3NPs) and to explore their potential use in combatting breast cancer cell lines (MCF-10) and liver cancer (HepG2). To achieve this, Nocardia bacteria, isolated from soil, were utilized. An extract was prepared by mixing equal volumes of the bacterial extract and gold tetrachloride, resulting in the creation of gold oxide nanoparticles (Au2O3NPs). The formation of NPs was ascertained using ultraviolet-visible spectroscopy (UV-VIS). Characterization of the synthesized Au2O3NPs was conducted through various techniques including transmission electron microscopy, scanning electron microscope, atomic force spectroscopy, and X-ray diffraction. After the synthesis and characterization, the cytotoxicity of the gold oxide nanoparticles was evaluated against human breast cancer cells MCF-7, human liver cancer cells HepG2, and normal mouse embryo cells MEF, using varying concentrations (100, 75, 50, 25, 12.5, 6.25 micrograms/ml). The data demonstrated a concentration-dependent activity of the particles against the mentioned cells, with the half-cell inhibitory concentration (IC50) of gold oxide nanoparticles derived from the Nocardia asteroides MT355849 indicating values of 46.84, 12.22, and 85.91 micrograms/ml against MEF, MCF-7, and HepG2 cell lines, respectively. Notably, the toxic effectiveness of the gold oxide nanoparticles on liver cells was found to be higher compared to breast cancer cells and normal cells. The results were further supported by statistical analysis performed using the Graph Pad Prism program, confirming the presence of significant differences between the inhibitory concentrations of cancer cells at a significance level of 0.05.