Antibacterial Activity of Reduced Graphene Oxide Nanoparticles Synthesized by Klebsiella Oxytoca
نویسندگان
1 Department of Biology, College of Science, Al-Muthanna University, Al-Muthanna, Iraq
2 Department of Biology- Microbiology, College of Science, University of Babylon, Microbiology, Babylon, Iraq
3 Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq
doi
10.22052/JNS.2023.04.023چکیده
The biological activity was studied using biogenic reduced graphene oxide nanocomposites (RGO) that combine some unique properties such as high surface area, conductivity, with biocompatibility, making them ideal for many medical and biological applications. The RGO nanocomposite were prepared from Klebsiella oxytoca (K. oxytoca) against the commonly occurring pathogenic bacterium Staphylococcus aureus (S. aureus) isolated from diabetic foot infections. The antibacterial activity of reduced graphene oxide nanocomposites against S. aureus was investigated at different concentrations. The synthesized nanoparticles were examined for antibacterial activity with 125, 250, 500, and 1000 µg/ml concentrations against pathogenic S. aureus. Reduced graphene oxide nanocomposites with these concentrations showed antioxidant activity by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. The inhibition percentage in the DPPH-reduced graphene oxide mixture was 29.42% at 125 µg/ml, 37.27% at 250 µg/ml, 40.76% at 500 µg/ml, and 54.06% at 1000 µg/ml. Reduced graphene oxide nanocomposites did not show any hemolysis for the tested blood. Additionally, biogenic reduced graphene oxide nanocomposites did not cause degradation of human DNA at the concentrations used in the current study.
