Evaluation of the Antibacterial Inhibitory Activity of Chitosan Nanoparticles Biosynthesized by Streptococcus thermophilus
نویسندگان
1 Alameen Center for Advanced Research and Biotechnology, Imam Ali Holy Shrine, Najaf, Iraq
2 Department of Biology, College of Science, University of Misan, Maysan, Iraq
3 AL Sader Teaching Hospital, Maysan Health Directorate, Ministry of Health, Maysan, Iraq
4 Department of Mechanical and Aerospace Engineering, Monash University, VIC 3800, Australia
5 Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq
doi
10.22052/JNS.2022.03.020چکیده
The nanomaterials field has been promising in recent decades due to its prospective biological applications that may assist overcome many medical difficulties by regulating numerous illnesses. However, there are restrictions on how these materials might perform against microbes including bacteria and viruses. Therefore. This research aims to investigate the nano synthesis of chitosan nanoparticles (NPs) using Streptococcus thermophilus, evaluate their characteristics, and analyze their antimicrobial efficiency against pathogenic bacteria associated with diabetic foot, Aeromonas hydrophila and Citrobacter freundii. By adding precursors to Streptococcus thermophilus cell-free supernatant, chitosan nanoparticles were produced. The solution's hue shift showed the production of chitosan NPs. UV-Visible spectrophotometry was utilized to characterize Streptococcus thermophilus' production of chitosan NPs with an absorption peak at 280nm. SEM examination indicated spherical, homogeneous, 29-51nm particles. EDS tests of biogenic chitosan indicated 33.31 % carbon, 10.24 % nitrogen, 50.55 % oxygen, 5.34% phosphate, and 0.55% chloride. Biogenic chitosan NPs were measured using XRD at 20°-26°. Energy-dispersive X-ray spectroscopy was utilized to determine the size, shape (spherical), dispersal (homogenous), and elemental analysis of nanoparticles. Biogenic chitosan NPs inhibit Aeromonas hydrophila and Citrobacter freundii. Biogenic chitosan NPs at 100, 200, and 400 ug/ml inhibited both tested microorganisms. Biogenic chitosan NPs showed growing antibiofilm activity. DPPH lowers biogenic chitosan nanoparticle activity.
