Carboxymethyl Chitosan Nano-Fibers for Controlled Releasing 5-Fluorouracil Anticancer Drug
نویسندگان
1 Departement of Chemistry, Faculty Mathematics and Natural Science, Hasanuddin University, Makassar,South Sulawesi, Indonesia
2 Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, Iraq
3 College of technical engineering, The Islamic University, Najaf, Iraq
4 Faculty of Biology and Ecology, Yanka Kupala State University of Grodno, Grodno, Belarus
5 Department of Occupational Therapy, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
6 Pharmaceutical Chemistry Department, College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq
7 College of technical engineering, The Islamic University, Najaf, Iraq
8 Department of Materials Engineering, Al-Nisour University, Baghdad, Iraq
9 Departemen of Nutrition, Faculty of Public Health, Universitas Airlangga, Indonesia
doi
0.22052/JNS.2022.01.013چکیده
In the present study, the pH responsive electrospun carboxymethyl chitosan nanofibers were prepared via electrospinning method and cross-linked with glutaraldehyde vapor for various times up to 48 h. The controlled release of 5-Fluorouracil (5-FU) from single layer and tri-layered nanofibers (5-FU in the middle layer) was compared to obtain a sustained delivery system of 5-FU anticancer drug. The release of 5-FU from nanofibers was investigated at 37 °C under acidic pH (pH 5.5) and physiological pH (pH 7.4). The release data were fitted by zero-order, Higuchi and Korsmeyer-Peppas pharmacokinetic equations to determine the 5-FU release mechanism from nanofibers. Tri-layered nanofibers exhibited the sustained delivery of 5-FU without initial burst release during 168 and 216 h at pH=5.5 and 7.4, respectively. The initial burst release followed by sustained release of 5-FU from single layer cross-linked carboxymethyl chitosan nanofibers occurred during 48 and 60 h. The “n” constant of Korsmeyer-Peppas equation indicated the non Fickian diffusion of 5-FU from single layer nanofibers at both pH values of 5.5, pH 7.4 and tri-layered nanofibers at pH 5.5. Whereas, the Fickian diffusion of 5-FU was occurred from tri-layered nanofibers at pH 7.4. The obtained results indicated the high capability of tri-layered nanofibers for controlled release of 5-FU compared to single layer nanofibers.
