High Performance Liquid Chromatography Method for Determination of Carnosine and Taurine and composition them with Fe3O4 nanoparticles
نویسندگان
1 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
2 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
3 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
4 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
5 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
6 Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia
doi
10.22052/JNS.2021.02.019چکیده
The present study reports the determination of the Fe3O4 nanoparticle based carnosine and taurine enzymes. The aim of the study was to develop a methodology that allows the determination of amino acids by HPLC in their joint presence in eye drops. In the result a method has been developed for the simultaneous quantitative determination of the components of eye drops (carnosine and taurine) by HPLC with preliminary preparation of dinitrophenyl (DNP) derivatives. This method has been validated for specificity, accuracy, precision, linearity, and analytical range, ranging from 80% to 120% of nominal. Nanotechnology is one of the quick rising areas of progressing technology, thus being a source of prospect for medicine and pharmacy. It is approved that, exactly metal oxides designed as nanoparticles will be used to soak medical devices and clothes, to fight viruses and bacteria, in new drug delivery systems or cancer therapy. For the medicinal science to completely profit from the new nanotechnology accomplishments, it is serious to determine the nanoparticles penetration mechanisms into the cells, their action inside the cells, the accumulation degree in various organs of living organisms. It has been cleared that numerous efforts at elucidating the biocompatibility of nano alumina for animals and humans.
