Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions

نویسندگان
Salavati-Niasari, M. ID 1
Shahbazkia, H. R. ID 2
Semnani, A. ID 3
Nooraei, P. ID 4
Shareghi, B. ID 5

1 Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O.BOX.87317-51167,I. R. of Iran

2 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

3 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

4 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

5 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

doi
10.7508/jns.2012.01.005
چکیده

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K stability and activity. Copper oxide nanoparticles had more inhibitory and destabilization effect than copper ions on proteinase K. We used thermodynamic parameter such as Tm and ∆G0  and kinetic studies to emphases the effect of nanoparticles CuO and copper ions on stability and activity of proteinase-K.

کلیدواژه‌ها
Proteinase-K, Copper oxide, Nanoparticles, Thermodynamic parameter, Nanostructures