Structural studies on the interaction of nano-SiO2 with lysozyme

نویسندگان
Shareghi, B. ID 1
Farhadian, S. ID 2
Salavati-Niasari, M. ID 3

1 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

2 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

3 Institute of Nano Science and Nano Technology, University of Kashan,Kashan,P.O.BOX.87317-51167,I. R. of Iran

doi
10.7508/jns.2011.03.004
چکیده

The interaction between nano-SiO2 and lysozyme was investigated by the method of UV-Visible  detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-SiO2 over the temperature range (293-373) K in different buffers and pH values, using temperature scanning spectroscopy. The presence of nano-SiO2 caused the destabilization of lysozyme resulting in a decrease in the temperature of unfolding with an increase in nano-SiO2 concentration.

کلیدواژه‌ها
Adsorption, Lysozyme, Nano-SiO2