Spectroscopic Studies on the Interaction of Nano-TiO2 with Lysozyme
نویسندگان
1 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
2 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
3 Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
4 Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O.BOX.87317-51167,I. R. of Iran
doi
10.7508/jns.2011.02.002چکیده
In the present study, the interaction between nano-TiO2 and lysozyme was investigated by the method of UV-Vis detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373) K in different buffer and pH, using temperature scanning spectroscopy. The presence of nano-TiO2 caused the destabilization of lysozyme resulting in a decrease in the temperature of unfolding with an increase in nano-TiO2 concentration.
