فهرست نشریات علمی دسترسی آزاد ایران

  Introduction: The main objective of this research was to study the chemical composition, as well as the antioxidant, anti-inflammatory, and hemolytic activities of the aerial part of the essential oil of Chrysanthemum coronarium and its isolated oxygenated fraction. Materials and Methods: The oxygenated fraction was isolated from the oil by column chromatography. Analyses by GC coupled with mass spectrometry allowed for the characterization of these components. The antioxidant properties of the essential oil and its oxygenated fraction were evaluated using the DPPH and FRAP methods. The anti-inflammatory effect was characterized by the denaturation of proteins. In addition, the hemolytic impact was studied through a suspension of erythrocytes in human blood. Results: The essential oil studied was characterized by a composition dominated by oxygenated monoterpenes (39.3%), followed by hydrocarbon sesquiterpenes (21.1%) and oxygenated sesquiterpenes (16.5%). The oxygenated fraction consisted mainly of perillyl alcohol (14.3%) and lyratyl acetate (14.5%). The results of the DPPH and FRAP tests showed significant antioxidant activity of the essential oil (IC50 = 3 ml/L) and the oxygenated fraction (IC50 = 4.4 ml/L), surpassing that of the synthetic antioxidant BHT. Additionally, the oxygenated fraction exhibited perfect anti-inflammatory activity, compared to essential oil and diclofenac sodium. The toxicity assessment on human erythrocytes shows that both essential oil and oxygenated fraction of Chrysanthemum coronarium have a very low hemolysis rate even at high concentrations. Conclusions: Tests on the essential oil and its oxygenated fraction have revealed promising properties. These results suggest promising opportunities for the development of new agents in the pharmaceutical field.