Optimization of Nisin and EDTA Concentration in Antimicrobial Film Based on Psyllium Seed Hydrocolloid by Response Surface Methodology
نویسندگان
1 Department of Green Technologies in Food Production and Processing, Research Institute of Food Science and Technology, Mashhad, Iran
2 Department of Chemical Engineering, College of Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran
3 Department of Green Technologies in Food Production and Processing, Research Institute of Food Science and Technology, Mashhad, Iran
doi
10.22101/JRIFST.2023.367074.1403چکیده
Nowadays, due to the environmental problems caused by packaging based on synthetic polymers, the use of producing edible films based on biopolymers like polysaccharides, proteins, and fats has been interested. According to all the useful features of psyllium seed hydrocolloid (PH), the aim of this study was to evaluate the possibility of antimicrobial biodegradable edible film production using pH, glycerol, nisin (0, 5000 and 10000 IU/g) and Ethylenediaminetetraacetic acid (EDTA), (0, 15 and 30 wt%). The diameter of the inhibition zones against pathogenic bacteria on medium surface were measured as antimicrobial activity of films. The results showed that films containing nisin and EDTA significantly inhibition of Staphylococcus aureus) and Salmonella typhimurium growth. With increasing concentration of nisin and EDTA the inhibitory power of film against pathogenic bacteria increased. Maximum inhibition was obtained at 3416.03 IU/g concentration of nisin and highest concentration of EDTA (30%). At the optimal point, the inhibition zone against S. aureus and S. typhimurium was 2.02±0.2 and 2.2±0.3 cm, respectively. Tensile and elongation of optimal film was 13.41 Mpa and 28.51%, respectively. According to results of this study, novel biodegradable antimicrobial PH film could be suggested as meat product packaging.
