Rapid Detection of Vibrio Cholerae by Polymerase Chain Reaction based on Nanotechnology Method
نویسندگان
1 Neuroscience Research Center, Baqiyatallah university of Medical Sciences, Tehran, Iran
2 Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3 Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4 Department of Biology, Payamnoor University, Tehran, Iran
5 Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
6 Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
7 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
8 Department of Biology, Payamnoor University, Tehran, Iran
doi
چکیده
Quick identification of Vibrio Cholerae in epidemics is important, on the other hand; conventional methods are time-consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitivity method for quick identification of Vibrio Cholerae. For this purpose we designed a PCR detection based on magnetic nanoparticles for identification of bacterial DNA by PCR Dynabead. So we used the biotinylated Probe for binding to DNA extracted from Vibrio Choleraeand other bacterial species (Salmonella, Shigella, Pseudomonas, E.coli) Using magnetic bead isolated with magnetic field, the Vibrio genome-specific primers (HlyA) for pathogen detection (PCR) was used. The results showed specific band was just for Vibrio Cholera (PCR positive); therefore designed probe was specific for Vibrio Cholerae. According to the findings, this study is characterized the high sensitivity of PCR using biotin-containing probes for DNA of Vibrio Cholera in contrast to the traditional methods.
