Phytochemistry and antioxidant activity of Lallemantia iberica aerial parts
نویسندگان
1 Department of Pharmacognosy, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.
2 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
3 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
4 Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
5 Institute of Medicinal Plants, Iranian Academic Center for Education, Culture & Research (ACECR),Karaj, Iran.
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چکیده
Background and objectives:Lallemantia iberica (Lamiaceae) is a medicinal plant distributed in different parts of Iran. This research, has evaluated the phytochemical constituents and antioxidant activity of the aerial parts of the plant. Methods: Different chromatographic methods such as column chromatographies using Silica gel (normal and reversed phases), Sephadex LH-20 and HPLC were used for isolation of the compounds from the ethyl acetate and methanol extract of L. iberica aerial parts. The structures of the isolated compounds were elucidated using 1H-NMR, 13C-NMR and EI-MS. Antioxidant activity of the extracts were also evaluated in DPPH and FRAP tests. Results: Two sterols,β-sitosterol acetate (1), β-sitosterol (2), one triterpenoic acid,ursolic acid (3), one polyphenol, rosmarinic acid (4) and six flavonoides,Luteolin-7-O-glucoside (5), 4'-methoxy-luteolin-7-O-glucoside (6), apigenin-7-O-glucoside (7), Luteolin (8), diosmetin (9), apigenin (10) were isolated and identified from the ethyl acetate and methanol extracts. The antioxidant activity of the ethyl acetate (IC50 189.95±2.8 μg/mL) and the methanol extracts (IC50 140±1.2 μg/mL) were compared to the standard antioxidant, BHA (IC50 100±1.6 μg/mL) in DPPH method. The reducing power of the ethyl acetate (300.28 μmol Eq FeSO4.7H2O/mg DW), the methanol extract (553.14 μmol Eq FeSO4.7H2O/mg DW) and BHA (558.36 μmol Eq FeSO4.7H2O/mg of standard) were elucidated in FRAP assay. Conclusion: The results introduce L. iberica as a medicinal plant with valuable constituents which are responsible of different pharmacological activities.
