Liquid Biopsy for Identifying hsa_Circ_0065173 and hsa_Circ_0008717 as Possible Biomarkers in Breast Cancer Patients Pre- and Post-Chemotherapy
نویسندگان
1 Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
2 Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
doi
10.22034/ircmj.2025.440232.0چکیده
Background and Objectives: Circular RNAs (circRNAs) are gaining attention as potential biomarkers and regulators in various biological processes, including cancer. Their role in breast cancer and chemotherapy response monitoring is particularly promising. This study investigates the expression profiles of hsa_circ_0008717/circABCB10 and hsa_circ_0065173 in the peripheral blood of breast cancer patients before and after chemotherapy, aiming to identify non-invasive possible biomarkers for treatment response. Methods: The expression levels of hsa_circ_0008717/circABCB10 and hsa_circ_0065173 were quantified in peripheral blood samples obtained from breast cancer patients using SYBR-Green Real-Time RT-qPCR before and after chemotherapy. Results: Both hsa_circ_0008717/circABCB10 and hsa_circ_0065173 were exclusively expressed in breast cancer patient’s blood. Elevated levels were significantly associated with lymph node involvement and metastasis (P<0.0001) and advanced disease stages. While hsa_circ_0008717/circABCB10 levels did not significantly change before and after chemotherapy (P=0.24), hsa_circ_0065173 expression decreased significantly post-chemotherapy (P<0.004). Conclusion: The expression of hsa_circ_0008717/circABCB10 and hsa_circ_0065173 in blood samples shows promise as diagnostic biomarkers and prognostic indicators for breast cancer. Notably, hsa_circ_0065173 appears to be a potential candidate for monitoring chemotherapy response.
