فهرست نشریات علمی دسترسی آزاد ایران

Background and Objectives: Sodium Nitroprusside (SNP), a nitric oxide donor and vasodilator, is employed in hypertensive emergencies, yet its impact on hemostasis, particularly under hyperthermic conditions, remains incompletely understood. This study investigates the influence of SNP and hyperthermia on primary hemostasis (platelet activation and aggregation) and secondary hemostasis (plasma clot formation).   Methods: Platelet rich plasma (PRP) and platelet poor plasma (PPP) samples which were prepared from whole blood of healthy volunteers, were added by sodium nitroprusside (SNP) in concentrations of 0, 10 and 100 μM, and then were incubated either at 37 ºC or 41 ºC. After 90 minutes of incubation, PRP samples were subjected to flow cytometry to determine CD62P expression on the membrane of ADP-stimulated platelets, as well as platelet aggregometry, using ADP as agonist. Before and after 90, 180, and 270 minutes of incubation, the PPP samples were subjected to prothrombin time (PT) and activated partial thromboplastin time (APTT) tests, to examine the clot formation step.   Results: Hyperthermia alone demonstrated a profound inhibition of platelet function, while SNP exhibited no significant mitigation of these effects. Unexpectedly, the combination of SNP and hyperthermia led to intense increases in clotting time levels, surpassing individual effects. Calculation of Combination Indices (CIs) suggested a high level of synergy.   Conclusion: Our findings reveal a complex interplay between SNP, hyperthermia, and hemostasis. Patient safety considerations highlight the need for cautious SNP administration in hyperthermic conditions.