Anti-Migration and Anti-Proliferation Effects of Recyclable Curcumin-Functionalized Silica Coated Fe3O4 Nanocomposite on LNCaP Prostate Carcinoma Cell Line
نویسندگان
1 Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
4 Department of Chemistry, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
doi
10.22034/ircmj.2024.445185.1029چکیده
Background and Objectives: Prostate cancer is the second most common malignancy in the men and the fifth leading cause of cancer death among men in the world. Curcumin as bright yellow and major active component from turmeric is a lipophilic polyphenol which have therapeutic properties such as anti-inflammatory, anti-oxidant and anti-cancer activities. Due to overcome its low solubility in water, in this study we designed and synthesized a magnetic nanocarrier for delivery of Curcumin to LNCaP prostate cancer cells and evaluated its therapeutic effects on cancer cells. Methods: In this study, the design and synthesis of Fe3O4@SBA-3 magnetic nanocomposites are described for the delivery of insoluble curcumin to LNCaP prostate carcinoma cells. The morphology and structure of Fe3O4@SBA-3@Curcumin MNCs were measured using FT-IR, FE-SEM, TEM, XRD, VSM, TGA, and EDX spectroscopy. The anti-proliferative and anti-migration properties of Fe3O4@SBA-3@Curcumin were evaluated by MTT assay, scratch test. The expression of some genes was assessed with Q-RT-PCR. Results: Our Physicochemical analysis showed that Fe3O4@SBA3-3 MNCs was functionalized with curcumin. TEM and FE-SEM images of MNCs showed particle sizes of ~60-92 nm. TGA analysis indicates high thermal stability (>300°C) of Fe3O4@SBA-3@Curcumin MNCs. IC50 of Curcumin in magnetic noncarriers was 61.04, 36.5, and 17.4 μg/mL after 24, 48, and 72h in LNCaP cell line. Scratch assay demonstrated that Fe3O4@SBA-3@Curcumin MNCs (61.04μg/ml) inhibited cancer cell migration. Q-RT-PCR analysis showed downregulation of demonstrated downregulation of TGFB1 and MMP2 and upregulation of apoptotic gene CASP9 and anti-invasive gene E-cadherin in Fe3O4@SBA-3@Curcumin MNCs (61.04 μg/ml) treated LNCaP cells after 24 hours. Conclusion: Our analysis suggested that Fe3O4@SBA-3@Curcumin MNCs, as a biocompatible and biodegradable nanocarrier without cytotoxicity effect, can be a therapeutic nanoparticle to delivery safe anticancer drugs such as curcumin.
