Anti-tumor Effect of the Ethanolic Extract of Vinca herbacea on Human Breast Cancer Cell Line: A Mechanism Study
نویسندگان
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of Advanced Therapy Medicinal Product, Breast Cancer Research Center, Motamed Cancer Institute, Academic Center for Education, Culture, and Research, Tehran, Iran Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran Immunotherapy Group, Institute of Pharmaceutical Science, Tehran University of Medical Science, Tehran, Iran
4 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
doi
10.32592/ircmj.2022.24.8.1922چکیده
Background: Breast cancer is extensively studied for its resistance to chemotherapy. Therefore, finding efficient therapeutic agents is vital to increasing the survival rate of patients. Objectives: Here, we conducted an in vitro study on the anti-tumor properties of Vinca herbacea extract on SKBR3 cells. Methods: Apoptotic and anti-invasive activity of V. herbacea extract was studied using Annexin FITC-V kit and Matrigel invasive assay, respectively. The expression of angiogenesis genes (VEGFR-1, VEGFR-2, and VEGF-A), apoptotic-related genes (Bcl-2 and BAX), as well as metastatic genes (MMP2 and MMP9) were studied using real-time polymerase chain reaction. Results: Vinca herbacea extract showed significant antiproliferative and cytotoxic effects on human breast cancer cells, compared to human embryonic kidney cells 293. Vinca herbacea extract had a great apoptosis-inducing potential in breast cancer cells by activating caspase-3 and increasing the BAX/Bcl-2 ratio. Vinca herbacea extract prevented cancer cell angiogenesis, marked by decreasing the expression level of angiogenesis-related genes, including VEGF, VEGFR-1, and VEGFR-2. In addition, V. herbacea extracts reduced cancer cell invasion and noticeably decreased the expression level of metastasis-associated genes, including MMP2 and MMP9. Conclusion: Vinca herbacea extracts exhibited vigorous cytotoxic effects on SKBR3 cells by the alteration of apoptosis, cell adhesion, invasion, and angiogenesis.
