Evaluation of Gene Expression Pattern of IL1B and IL10 Cytokines Following Vitamin C Administration among Brain-Dead Liver Donors
نویسندگان
1 Departments of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, IR Iran
2 Anesthesiology and Critical Care Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
3 Departments of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, IR Iran
4 Anesthesiology and Critical Care Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
5 Maternal-Fetal Medicines Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
doi
چکیده
Inflammatory events following brain death (BD) decrease the quality of donor organs which can affect the outcomeof the transplantation. It experimentally and clinically is verified that the level of inflammatory cytokines is increased in the BD ofthe donor results. In experiments, the in vitro use of vitamin C can successfully decrease the levels of inflammatory cytokines.Objectives: The aim of this study was to investigate the influence of vitamin C administration on the inflammatory status of thegraft in BD liver donors.Methods: This study was conducted at Nemazee Hospital’s transplant center (Shiraz, Iran). In this interventional study, the BDliver donors (n = 40) were randomly divided into two groups. The control group received only the routine intensive care unit (ICU)considerations, and the intervention group was treated with Vitamin C before harvesting organ (100 mg/kg, initially followed by100 mg/kg/6h until organ removal). Blood samples were taken from BD patients in the intervention and control groups 3 times: 6hours before operation, immediately after laparotomy, and immediately prior to clamping the aorta to assess the gene expressionratio of Interleukin 1 beta (IL1B) and Interleukin 10 (IL10) cytokines using real time polymerase chain reaction (PCR) (n = 40). Solubleserum cytokines were measured by enzyme-linked immunosorbent assay (ELISA) (n = 24).Results: No significant differences were observed in mRNA expression ratio of IL1B and IL10 between two groups. Despite an acceptabledecrease in serum concentration of the inflammatory markers IL1B and IL10 at time point 2 minus time point 1 (T2-1), nosignificant differences were observed in vitamin C-treated BD donors compared with the control donors. There was a significant differenceamong aspartate aminotransferase (AST) and alanine aminotransferase (ALT) changes on the 3rd to 1st days after operationbetween the control and intervention recipients (P < 0.05).Conclusions: The present study suggests a beneficial effect of vitamin C adsministration on post-transplant function of the liverfrom BD liver donors.
