Dose–Response Curves of the FDXR and RAD51 Genes with 6 and 18 MV Beam Energies in Human Peripheral Blood Lymphocytes
نویسندگان
1 Department of Radiology and Nuclear Medicine, Paramedical School, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
2 Department of Virology, Faculty of Medicine, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, IR Iran
3 Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran
4 Department of Medical Genetics, Faculty of Medicine, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, IR Iran
5 Department of Radiology and Nuclear Medicine, Paramedical School, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
doi
چکیده
Background: Rapid dose assessment using biological dosimetry methods is essential to increase the chance of survival of exposedindividuals in radiation accidents.Objectives: We compared the expression levels of the FDXR and RAD51 genes at 6 and 18 MV beam energies in human peripheralblood lymphocytes. The results of our study can be used to analyze radiation energy in biological dosimetry.Methods: For this in vitro experimental study, from 36 students in the medical physics and virology departments, seven voluntary,healthy, non-smoking male blood donors of Khuzestan ethnicity with no history of exposure to ionization radiation were selectedusing simple randomized sampling. Sixty-three peripheral blood samples were collected from the seven healthy donors. Humanperipheral bloodwasthen exposed to doses of 0, 0.2, 0.5, 2, and4Gywith6and18MVbeamenergies in a LinacVarian 2100C/D (Varian,USA) at Golestan hospital in Ahvaz, Iran. After RNA extraction and cDNA synthesis, the expression levels of FDXR and RAD51 weredetermined 24 hours post-irradiation using the gel-purified reverse transcription polymerase chain reaction (RT-PCR) techniqueand TaqMan strategy (by real-time PCR).Results: The expression level of FDXR genewassignificantly increased at doses of 2Gyand4Gyin the 6 - 18MVenergy range (P< 0.001and P < 0.008, respectively). The medians with interquartile ranges (IQRs) of the copy numbers of the FDXR gene at 2 Gy and 4 Gydoses under 6 and 18 MV beam energies were 2393.59 (1798.21, 2575.37) and 2983.00 (2199.48, 3643.82) and 3779.12 (3051.40, 5120.74)and 5051.26 (4704.83, 5859.17), respectively. However, RAD51 gene expression levels only showed a significant difference betweensamples at a dose of 2 Gy with 6 and 18 MV beam energies, respectively (P < 0.040). The medians with IQRs of the copy numbers ofthe RAD51 gene were 2092.77 (1535.78, 2705.61) and 3412.57 (2979.72, 4530.61) at beam energies of 6 and 18 MV, respectively.Conclusions: The data suggest that the expression analysis of the FDXR gene, contrary to that of the RAD51 gene, may be suitable forassessment of high-energy X-ray. In addition, RAD51 is not a suitable gene for dose assessment in biological dosimetry.
