Accelerated BurnWound Closure in Mice with a New Formula Based on Traditional Medicine
نویسندگان
1 1Department of Pediatrics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, 2Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran,
2 Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran
3 Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, IR Iran
4 Department of Pathology, Kerman University of Medical Sciences, Kerman, IR Iran
5 Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IR Iran
6 Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
7 Faculty of Medicine, Hazrat-e-Rasoul Hospital, Tehran University of Medical Sciences, Tehran, Iran
8 Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
9 Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran
10 Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, IR Iran
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چکیده
Background: A combination of the oils of sesame, hemp, wild pistachio, and walnut has been used for treatment of skin disorders,including wound burns, in some parts of Kerman, Iran. Evaluation of this remedy in the form of a pharmaceutical formulation inanimal models can pave the way for its future application in wound burn healing in humans.Objectives: This experimental study investigated the healing potential of a new formula (NF) based on folk medicine from Iran forthe treatment of third degree burns in mice. The formula was a combination of the oils of four plants: sesame (Sesamum indicum L.),wild pistachio (Pistacia atlantica Desf.), hemp (Cannabis sativa L.), and walnut (Juglans regia L.)Methods: Twenty-four mice were selected based on simple random sampling. Twenty-five percent of the total body surface area wasburned by exposure to boiling water, according to theWalker-Mason method. NF and silver sulfadiazine (the positive control) wereapplied topically twice a day for 21 days. The burned area in the negative control group was left untreated. Epithelialization timeand the percent of wound contraction were measured during the treatment period. The process of wound repairing was evaluatedusing histological (H and E and trichrome staining) and immunohistological (anti-pancytokeratin) methods.Results: Whencompared to the controls, NF significantly improvedwoundcontraction after day 10. Epithelialization time in the NFgroup was significantly faster than in the other groups (20 vs. 25.5 days) (P < 0.001). Histopathological and immunohistochemicalfindings confirmed the efficacy of the NF.Conclusions: A new therapeutic remedy was introduced for the treatment of burn wounds. Further clinical and molecular studiesare suggested to determine the exact mechanism(s) involved in the burn wound healing effect of NF.
