MiR-328 May be Considered as an Oncogene in Human Invasive Breast Carcinoma
نویسندگان
1 Department of Medical Genetics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Medical Physics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Medical Physics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Medical Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5 Modeling in Health Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
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چکیده
The recent investigations have rendered microRNAs (miRs) as a novel biomarker in cancer research. In fact, alterationin miR expression may be associated with tumor suppression, tumorigenesis, metastasis, and poor prognosis in human breast cancer(BC).Objectives: The aim of this clinical experimental study was to measure the miR-328 expression level in breast cancer tissues, at first.Then, we tried to find out any possible correlation between miR-328 and prognostic and predictive biomarkers in BC. Both of thesetwo objectives were investigated for the first time; and we did not find any similar survey measuring the expression level of miR-328in both tumor and non-tumor breast tissues. This research was conducted in Iran (Ahvaz, Khuzestan), between December 2013 andApril 2014. Furthermore, we did not find any previous document investigating the correlation between miR-328 expression leveland prognostic factors in BC. Due to the lack of similar studies intending to measure the expression level of miR-328 in tumor andadjacent non-tumor tissues, we decided to carry out a pilot study.Methods: We measured the expression level of miR-328 by Poly (A) real-time PCR based on SYBR Green-I in 28 fresh samples of BCtissues and 28 samples of normal adjacent tissues, including invasive ductal carcinoma (IDC), invasive lobular carcinoma (ILC), andductal carcinoma in situ (DCIS).We tried to attribute the results to clinicopathologic features such as status of estrogen and progesteronereceptors (ER/PR), HER2/neu (HER2), P53 and also Ki67 labeling (Ki67-LI).Results: The results showed that the miR-328 median level of expression was 0.88 (2-Ct) (25th-75th percentile, 0.07 - 2.34). It meansthat the expression level increased in tumor tissues compared to normal adjacent tissues (NATs). However, a statistically significantcorrelation between the miR-328 median expression level and prognostic factors, including pathologic diagnosis, age, and also thestatus of ER, PR, HER2, and Ki67-LI was not observed (P > 0.05).Conclusions: Therefore, it might be possible to consider miR-328 as an oncogene; but not necessarily an oncomiR, in human BC.
