Angiotensin II Type 1 Receptor Gene A1166C PolymorphismWas Not Associated With Acute Coronary Syndrome in an Iranian Population
نویسندگان
1 Cardiovascular Research Center, Avicenna Research institute, Mashhad University of Medical Sciences, Mashhad, IR Iran
2 Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
3 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
4 Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
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6 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
7 Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
8 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
9 Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
10 Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran
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چکیده
There are very limited data for Iranian populations on the predisposing genetic factors for acute coronary syndrome(ACS).Objectives: The objective of the present study was to investigate the association of the angiotensin II type 1 receptor (AT1R) genepolymorphism and ACS in an Iranian population.Patients and Methods: This cross-sectional study was conducted among 263 subjects (97 men and 166 women). Patients (n = 128)aged 30 - 80 years with chest pain were recruited from the emergency department of Ghaem Hospital (Mashhad, Iran). A 12-leadelectrocardiograph plus creatine kinase MB (CK-MB) levels were used as the basis for the diagnosis of myocardial ischemia. Thecontrol group was selected from age-matched healthy subjects (n = 135). Non-enzymatic kits were used for extraction of DNA fromblood samples. Polymerase chain reaction (PCR) was performed to amplify the DNA fragments. For restriction fragment lengthpolymorphism (RFLP) determination, the DdeI enzyme was used to digest the amplified DNA fragments. Statistical analyses wereperformed using SPSS version 13.0.Results: There wasnostatistical difference in the genotype frequency of patients and healthy subjects with regard to age and gender(P > 0.05).Conclusions: The AT1R A1166C polymorphism appeared not to be associated with the presence of ACS in the population studied.
