فهرست نشریات علمی دسترسی آزاد ایران

Valproic acid (VPA)andcarbamazepine (CBZ),twowidely used antiepileptic drugs, have recentlybeenfound to inhibithistone deacetylases (HDAC). HDAC inhibitors (HDACIs) have various effects on cancer cells.Objectives: The aim of this study was to compare the anticancer activity of these drugs on SW480 colon cancer cell lines.Methods: In the present experimental study, implemented during 2014 - 2015 in Iran, after incubation of cells into 96-well plateswith 5,500 cells/well, the tested drugs were added, and cytotoxic effects were assessed by MTT. Moreover, after incubation of 8106cells in 75 cm2 flasks to obtain  -catenin levels and 106 cells in a six-well plate to obtain vascular endothelial growth factor (VEGF)levels , these levels were estimated using enzyme-linked immunosorbent assay (ELISA) analysis.Results: Through MTT assay, we found that the inhibitory concentration of 50% (IC50) values for VPA and CBZ were 2.5 mM and 5M, respectively in comparison to controls in terms of total concentration and times evaluated (P < 0.0001). We also found thattreatments with these drugs decreased levels of  -catenin (P < 0.0001) and VEGF (P < 0.0001) significantly more than controls.Conclusions: VPA and CBZ treatments caused a decrease in -Catenin and VEGF levels in SW480 colon cancer cell lines. These resultssuggest that CBZ can be considered a potential antitumor drug with potencies different from VPA.