The Effects of Monosodium Glutamate and Tannic Acid on Adult Rats
نویسندگان
1 Department of Medical Biology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
2 Department of Medical Biology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
3 Department of Medical Biology, Istanbul Aydin University, Faculty of Medicine, Istanbul, Turkey
4 Department of Histology and Embryology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
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6 Department of Medical Biology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
7 Department of Medical Biology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
8 Department of Medical Biology, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
9 Department of Medical Biology, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, Turkey
10 Department of Biostatistics, Eskisehir Osmangazi University, Faculty of Medicine, Eskisehir, Turkey
11 Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey
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چکیده
Monosodium glutamate (MSG) is a widely-used flavor enhancer and stabilizer in ready-made or packaged foods. Theexcessive use of MSG has been shown to increase oxidative stress in different organ systems and causes glucose metabolism disorders,obesity, and coronary diseases.Objectives: In this study, the antioxidant activity of tannic acid was investigated experimentally with respect to its protective effectsagainst overdosed MSG-induced oxidative stress in rats. The study took place in Turkey in August 2013.Methods: Four groups (n = 7) of three- to four-month-old Sprague-Dawley female rats were used in this study. The first group was thecontrol, who were administered saline. The second group received tannic acid (50 mg/kg, 3 days) intraperitoneally (i.p.). The thirdgroup received MSG (2 g/kg, 7 days) i.p., and the fourth group received both tannic acid (50 mg/kg, 3 days, pretreatment) and MSG(2 g/kg, 7 days) i.p. The animals were euthanized ten days later. Blood was collected for determining the hematological values andblood glucose levels. Superoxide dismutase (SOD)andmalondialdehyde(MDA)levels were determined in the brain, liver, andkidneyhomogenates, and in the erythrocyte hemolysate. Histopathological examination of the brain, liver, and kidneys was conductedthrough hematoxylin-eosin staining.Results: The data showed that the tannic acid treatment statistically decreased the MDA levels in the brain tissues of the groupadministered MSG and tannic acid (P < 0.001) when compared to the corresponding values of the control group. The SOD activitiesin the blood hemolysates of theMSGandtannic acidgroupincreasedwhencomparedto the corresponding values for theMSGgroup(P < 0.01). Additionally, we found that pretreatment with tannic acid reduced blood glucose levels in comparison to the levels ofthe MSG group (P = 0.029). The results of our study show that tannic acid pretreatment in adult rats decreased blood glucose levelsand oxidative stress.Conclusions: In the literature, it was observed that short-term MSG exposure does not cause significant histological changes in thekidneys, liver, or brain cortex. These findings should be re-evaluated in additional long-term studies.
