فهرست نشریات علمی دسترسی آزاد ایران

The current medical treatment for hepatitis C virus (HCV) infection is pegylated interferon plus ribavirin, but just50% of genotype 1 HCV patients and about 80% of HCV genotype 3 patients are treated completely. Recently, the rs12979860 C/Tpolymorphism, which is located 3 kb upstream of the IL28b gene that codes IFN3, shows a powerful association in response to thetreatment in HCV patients.Objectives: The aim of this study was to evaluate the relationship between IL28b single nucleotide polymorphism (SNP) and treatmentoutcomes among chronic HCV patients in Iran.Patients and Methods: In this cross-sectional study, 108 blood samples were collected from chronic patients in Iran; 50 unrelatedhealthy subject samples were also collected. Genomic DNA was extracted, and rs12979860 SNP was done by PCR-RFLP. Finally, productswere detected on 12% polyacrylamide gel electrophoresis.Results: The analysis of data for C/T SNP showed that the CC genotype is more common in the control group than in the group ofpatients. In contrast, the frequency of TT as a mutant genotype is more frequent in patients than in uninfected people. In addition,results showed a statistically significant relationship between CC, CT, and TT genotypes in sensitive and resistant groups (P value: <0.001, Or: 0.003, CI: 0-0.047). This relationship was also examined in terms of allele frequency, to determine whether the possibilityof resistance to treatment in patients with T allele is more than in patients who carry C allele (P value: < 0.001).Conclusions: These results showed a significant effect between rs12979860 SNP and sustained virological response (SVR) rate inIranian patients with chronic HCV. To decrease the cost of long treatments and to prevent severe side effects, determining this polymorphismat the beginning of treatment can be very helpful for patients and physicians.