Coenzyme Q10 Ameliorates Trimethyltin Chloride Neurotoxicity in Experimental Model of Injury in Dentate Gyrus of Hippocampus: A Histopathological and Behavioral Study
نویسندگان
1 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IR Iran
2 Neurophysiology Research Center, Department of Anatomical Sciences, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Oncopathology Research Center, Iran University of Medical Sciences, Tehran, IR Iran
4 Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, IR Iran
5 Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, IR Iran
6 Cellular and Molecular Research Center, Faculty of Advanced Technologies in Medicine, Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, IR Iran
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چکیده
Coenzyme Q10 has antioxidative and free radical scavenging effects. CoQ10 supplementation is known to have neuroprotectiveeffects in some neurodegenerative diseases, such as Parkinson’s disease and Huntington’s disease.Objectives: The aim of this study was to evaluate both histopathologic and behavioral whether Coenzyme Q10 is protective againsttrimethyltin chloride (TMT) induced hippocampal damage.Materials and Methods: This was an experimental study. Thirty-six Balb/c mice were divided into four groups, as follows: 1) controlgroup; 2) sham group of mice that received a 100 L intraperitoneal injection (IP) of sesame oil; 3) TMT group of mice that receiveda single 2.5 mg/kg/day IP injection of TMT; and 4) TMT + CoQ10 group of mice that received a 10 mg/kg IP injection of CoQ10. Bodyweight and Morris water maze (MWM) responses were investigated. In addition, the dentate gyrus neurons of the hippocampuswere evaluated histopathologically by light and electron microscopes.Results: This study revealed that the body weight scale was found to be significantly higher in the CoQ10 group (21.39 2.70),compared to the TMT group (19.392.74) (P < 0.05). In the TMT group, the animals showed body a weight loss that was significantlylower than that of the control group (22.33 3.06) (P < 0.05). Our results showed that CoQ10 provided protection against MWMdeficits. Furthermore, TMT impaired the ability of mice to locate the hidden platform, compared to the control group (P < 0.05).Microscopic studies showed that TMT caused histopathological changes in the dentate gyrus and increased the number of necroticneurons (47678.51), compared to the control group (20840.84) (P < 0.001). But, CoQ10 significantly attenuated (31 960.08)the density of necrotic neurons compared to TMT (P < 0.05).Conclusions: The results of the present study indicate that Coenzyme Q10 diminished neuronal necrosis and improved learningmemory. Part of its beneficial effect is due to its potential to discount oxidative stress.
