Protective Effects of Co-Enzyme Q10 on Thioacetamide-Induced Acute Liver Damage and Its Correlation With Behavioral, Biochemical, and Pathological Factors
نویسندگان
1 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
2 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
3 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
4 Organ Transplant Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran
5 Department Of Pharmacology, Fasa University of Medical Sciences, Shiraz, IR Iran
6 Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, IR Iran
7 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
8 Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IR Iran
9 International Branch, Shiraz University of Medical Sciences, Kish, IR Iran
10 Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, IR Iran
doi
چکیده
Acute liver damage may be followed by biochemical, behavioral, and pathological alterations, which can result inserious complications and even death.Objectives: In this experimental studywedetermined whether coenzymeQ10 (CoQ10), acommonsupplementary medicine knownto have protective, antioxidative, and anti-inflammatory effects in cells, has any protective effect against thioacetamide (TAA)-induced liver damage and its related neurobehavioral alterations in rats.Materials and Methods: In this experimental study forty-eight Wistar rats were divided randomly into four groups (n = 12): C1was the control group; C2 received a single-dose of TAA (350mg/kg; intraperitoneally) without any other treatment; E1 received TAA+ 5 mg/kg CoQ10 (intraperitoneally); and E2 received TAA + 10 mg/kg CoQ10. After sacrificing the rats, liver enzymes and plasmaammonia(NH4) were measured and histopathological analyses of the livers were carried out. Elevated-plus-maze, open-field, andforced-swimming tests were also performed to investigate behavioral correlations.Results: The serum levels of alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), and NH4 show significant increases(P< 0.05). The groups treated withCoQ10wereshowntohave significantly lower clinical grade of encephalopathy (P=0.001), higherlocomotor activity (P = 0.000), and lower levels of depression (P = 0.000). Furthermore, it was also shown that CoQ10 treatmentmaylead to significant decreases in scores of centrilobular necrosis, apoptosis, inflammatory cell infiltration, vacuolization, and livernecrosis (P < 0.05).Conclusions: Overall, CoQ10 was determined to have positive effects on liver injury and its related behavioral and biochemicalchanges.
