Phytochemical Analysis and Antioxidant Activity of Salvia chloroleuca Aerial Extracts
نویسندگان
1 Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, IR Iran
2 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, IR Iran
4 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR Iran
doi
چکیده
Background: Salvia, knownasMaryamGoli in the Persian language, is an important genus that includes approximately900speciesin the Lamiaceae family. There are 58 Salvia species growing naturally in Iran, including Salvia chloroleuca Rech. f. and Allen., whichgrows wild in the northeastern and central parts of the country.Objectives: This study was designed to determine the chemical composition, in vitro antioxidant activity, and total phenol contentof various extracts of S. chloroleuca.Materials and Methods: Dried aerial parts of the plant were crushed, then sequentially extracted with n-hexane, ethyl acetate, andmethanol. The fractions of S. chloroleuca were subjected to silica gel column chromatography and Sephedex LH-20. The antioxidantactivities of these extracts were measured by ferric reducing antioxidant power (FRAP), and the total phenolic contents of theextracts were evaluated using Folin-Ciocalteu reagent.Results: The separation and purification processes were carried out using different chromatographic methods. Structural elucidationwasonthe basis 1H-NMRand 13C-NMR spectral data, in comparison with that reported in the literature. The isolatedcompoundswere salvigenin (1), luteolin (2), cirsiliol (3), -sitosterol (4), and daucosterol (5). Ethyl acetate extract displayed the highest level oftotal antioxidants and total polyphenols compared to the other analyzed extracts (n-hexane and methanol). In the FRAP assay, ethylacetate extract had the highest (230.410.5) FRAP value, followed by methanol (211.48.3) and n-hexane (143.412.04). Total phenolcontents were calculated to be 13.8 0.3, 58.25 0.05, and 43.48 0.38 mg of gallic acid/100 g in the n-hexane, ethyl acetate,and methanol extracts, respectively.Conclusions: The above-mentioned compounds were isolated for the first time from S. chloroleuca. The antioxidant activity of thisplant could be in part related to isolated flavonoids and sterols. The results of this study indicated that S. chloroleuca could be animportant dietary source of phenolic compounds with high antioxidant capacity.
