Effects of Acellular Amniotic Membrane Matrix and Bone Marrow-Derived Mesenchymal Stem Cells in Improving Random Skin Flap Survival in Rats
نویسندگان
1 Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR Iran.
2 Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR Iran
3 Department of Physical Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran
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6 Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR Iran
7 Department of Biostatistics, Faculty of Paramedicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
doi
چکیده
Background: The necrotic skin flap represents a great challenge in plastic and reconstructive surgery. In this study, we evaluatedthe effect of bioscaffolds, acellular amniotic membranes (AAMs), and bone marrow-derived mesenchymal stem cells (BM-MSCs) onrandom skin flap (RSF) survival in rats by applying a cell-free extracellular matrix scaffold as a supportive component for the growthand proliferation of BM-MSCs on RSFs. AAM matrix scaffolds were created by incubating AMs in ethylenediaminetetraacetic acid0.05% at 37°C, and cell scrapers were used.Objectives: The aim of the present study was to assess the effect of AAM as a scaffold in TE, and combined with transplanted BMMSCs,on the survival of RSFs and on the biomechanical parameters of the incision-wound flap margins 7 days after flap elevation.Materials and Methods: BM-MSCs and AAMs were transplanted into subcutaneous tissue in the flap area. On the 7th postoperativeday, the survivingflapareas weremeasuredusing digital imaging software, andtheflaptissuewascollected for evaluation. Forty ratswere randomly divided into four groups of 10 each: group 1 received an AAM injection; group 2 underwent BM-MSC transplantation;group 3 received both AAM injection + BM-MSC transplantation; and group 4 was the control group, receiving only saline.Results: The survival area in the AAM/BM-MSC group was significantly higher than in the control group (18.491.58 versus 7.512.42, P < 0.05). The biomechanical assessment showed no significant differences between the experimental groups and the controlgroup (P > 0.05), and there was no correlation with flap survival.Conclusions: Our findings showed that the treatment of flaps with BM-MSC and AAM transplantations significantly promoted flapsurvival compared to a control group. The viability of the flap was improved by combining BM-MSCs with AAM matrix scaffolds.
