Haplotype Analysis of Hemochromatosis Gene Polymorphisms in Chronic Hepatitis C Virus Infection: A Case Control Study
نویسندگان
1 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IR Iran
2 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IR Iran
3 Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
4 Department of Biostatistics, Public Health School, Mashhad University of Medical Sciences, Mashhad, IR Iran
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چکیده
Background: Chronic hepatitis C virus (HCV) infection is frequently associated with elevated serum iron markers. Polymorphismsin the hemochromatosis (HFE) genes are responsible for iron accumulation in most cases of hemochromatosis, and may play a rolein HCV infection.Objectives: We aimed to assess the prevalence of HFE gene polymorphisms in a group of Iranian HCV-infected patients, and toexplore the association of these polymorphisms with HCV infection.Patients and Methods: HFE gene polymorphisms were examined in a total of 69 HCV patients and 69 healthy controls using polymerasechain reaction and restriction fragment length polymorphism techniques. Haplotype and diplotype analyses were performedusing PHASE software.Results: In a recessive analysismodelof the His63Asp (H63D) locus(HHvs. HD+DD), theHHgenotypewasmorecommonin patientscompared to controls (adjusted P = 0.012; OR = 6.42 [95% CI: 1.51 - 27.33]). Also, in a recessive analysis model of the Cys282Tyr (C282Y)locus (CC vs. CY + YY), the CC genotype was more frequent in patients compared to controls (adjusted P = 0.03; OR = 5.06 [95% CI: 1.13- 22.06]). In addition, there was a significant association between the HC haplotype and the HCDC diplotype and HCV infection.Conclusions: Polymorphism in the hemochromatosis gene may confer some degree of risk for HCV infection, and individuals carryingthe H and C alleles may be susceptible to this disease; however, a larger sample of HCV patients and healthy individuals maybe necessary to further illustrate the role of these polymorphisms in HCV.
