Xenotropic Murine Leukemia Virus-Related Virus and RNase L R462Q Variants in Iranian Patients With Sporadic Prostate Cancer
نویسندگان
1 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
2 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
3 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
4 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
5 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
6 Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
7 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
8 Department of Statistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
9 Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IR Iran
10 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
doi
10.5812/ircmj.19439چکیده
Background: Although several studies have confirmed the association of xenotropic murine leukemia virus-related virus (XMRV) and prostate cancer, this association is still controversial, as most studies did not detect XMRV in prostate tissue samples. Furthermore, some genetic and epidemiological studies have highlighted a role for RNase L polymorphisms, particularly R462Q, in the progression of prostate cancer.Objectives: The focus of this study was on the association of XMRV and RNase L R462Q variants with the risk of prostate cancer in Iranian patients.Patients and Methods: In this case-control study, 40 and 80 individuals with sporadic prostate cancer and benign prostatic hyperplasia, respectively, were included. The presence of XMRV was evaluated by real-time polymerase chain reaction (PCR) of integrase and nestedPCR for the gag genes. The RNase L R462Q polymorphism analysis was carried out by PCR and sequencing.Results: In a total of 40 sporadic prostate cancer and 80 benign prostatic hyperplasia cases, no XMRV was detected by real-time PCR and nested-PCR. RNase L R462Q polymorphism analysis reveals that although there was an increase in the risk of prostate cancer correlated with the Q/Q allele of RNase L at position 462, the frequencies of the RNase L R462Q alleles were not statistically significant between the prostate cancer and benign prostatic hyperplasia groups (OR = 2.75 (95% CI = 0.67 - 11.3), P = 0.29).Conclusions: These results did not support the presence of XMRV in the samples with prostate cancer and showed that RNase L R462Q variants had relatively little or no impact on the risk of prostate cancer in Iranian population.
