Detection of Drug Resistance Gene in Cutaneous Leishmaniasis by PCR in Some Endemic Areas of Iran
نویسندگان
1 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan,
2 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan,
3 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan,
4 Cellular and Molecular Biology Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
5 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan,
6 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan,
7 Cellular and Molecular Biology Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, Iran
doi
چکیده
Background: Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drugresistance has emerged as a major impediment in the treatment of leishmaniasis. This study aims to determinethe drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran.Methods: Ninety seven samples were collected from ulcers of leishmaniasis patients from some endemic areasof Iran. The Giemsa stained samples were examined microscopically and cultured in NNN and RPMI 1640 mediumsfor parasite detection. After DNA extraction, PCR was done by a pair of specific primers. For detection ofmutation in DNA, first PCR products were electrophoresed on CSGE gel. The suspected samples were comparedby sequencing and RFLP results were demonstrated. Comparison of DNA derived from a wild type celland mutant cell was undertaken by CSGE and sequencing methods.Results: Among 90 isolates (92.8%) examined for detection of mutation in gene with CSGE and RFLP, 10(11.1%) revealed a disorder in sequencing selection for unresponsive to drug.Conclusion: Drug resistance in cutaneous leishmaniasis to sodium stiboglocanat is probably due to a mutationin a genome. A field study is needed to determine the distribution of drug resistance and other gene mutationsinvolved in unresponsiveness to drugs in leishmaniasis endemic areas of Iran.
