Effects of Low Intensity Pulsed Ultrasound on Healing of Denervated Tibial Fracture in the Rabbit
نویسندگان
1 Trauma Research Center
2 Applied Neuroscience Research Center,
3 Department of Anatomy, Army University of Medical Sciences, Tehran, Iran
4 Department of Anatomy, Baqiyatallah University of Medical Sciences,
5 Department of Biophysics, Tarbiat Modares University,
doi
چکیده
Background: Low intensity pulsed ultrasound (LIPUS) has been shown in healing of fractures. This study investigatesthe effect of LIPUS as a fracture-healing adjunct on the denervated tibial fractures in rabbit model.Methods: Twenty four male Dutch rabbits were randomly divided into four groups including two control and twoultrasound-treated (US-treated) groups. A standardized mid-tibial osteotomy was performed in the right tibia.Ipsilateral sciatic nerve was cut two centimeter above the right knee. After three days, the rabbits of US-treatedgroups received the therapeutic dose (100 mW/cm²) of ultrasound for 15 minutes per day until the 6th and 8thweek and the control groups did not receive any treatment. The rabbits were sacrificed at weeks 6th and 8th postsurgery and the external fixator and tibia were carefully removed. Radiographs were taken by lateral and anteroposteriorviews. Mechanical testing of rabbit’s tibiae was used. The specimens were taken from callus of fracturefor histomorphometric study of trabecula in the fractured area. Immunohistochemical staining for ki67 was usedto evaluate cellular proliferation.Results: Radiographs showed the callus was visible indicating the ossification of the callus tissue without anysignificant difference between US- treated and control groups. Biomechanical, histomorphic and immunohistochemicalevaluations had no significant difference between control and US-treated groups after 6 and 8 weekspostoperation.Conclusion: These finding suggest that ultrasound therapy with features which applied in this study had nosignificant effect on denervated tibial fracture repair in rabbits.
