فهرست نشریات علمی دسترسی آزاد ایران

Discovered by Alexander Fleming in 1928, penicillin has earned its reputation as a revolutionary antibiotic, profoundly altering the treatment of bacterial infections and marking a pivotal moment in medical history. Penicillium chrysogenum, a high-yielding fungal species, remains central to the industrial production of penicillin. Efforts to improve the yield of penicillin remain an active area of research, with different approaches, such as induced mutagenesis, applied to maximize production efficiency. This study aims to investigate the effects of induced mutations and the utilization of various carbon sources on penicillin biosynthesis in Penicillium chrysogenum. Mutant strains developed through gamma radiation exposure were evaluated against wild-type strains for antibiotic production in culture media containing lactose, sucrose, and various combinations of both at different concentrations. Penicillin production was quantified using high-performance liquid chromatography (HPLC), with 96 analyses conducted in triplicate. The study found a marked increase in penicillin production in mutant strains compared to wild-type strains, particularly in sucrose media. However, the relationship between antibiotic yield and carbon source concentration was nonlinear. Increasing carbon source concentration was not always associated with increased penicillin production. The research suggests optimizing carbon source concentration and mutation methods to enhance penicillin production. It has implications for the schematic development of bioprocesses for the production of industrial antibiotics.