فهرست نشریات علمی دسترسی آزاد ایران

During a study on the biodiversity of ascomycetous micro-fungi from 2020 to 2024, several fungal isolates from healthy Cannabis seeds (Cannabis sativa L.) and healthy Camelthorn roots (Alhagi maurorum Medik), as endophytic fungi, and some of them from laboratory air, as airborne fungi, were recovered and characterized. Based on the morphological investigations, part of the identified fungi belonged to the genus Microascus (Microascaceae). Molecular phylogenetic analysis using the partial nucleotide sequence of the β-tubulin (tub2) gene and the resulting consensus phylogenetic tree revealed that the recovered isolates belong to three different Microascus species, including M. cinereus from healthy Camelthorn root, M. croci from laboratory air and M. trigonosporus from healthy Cannabis seed with high levels of sequence similarity with the relevant sequences from GenBank. In the resulting consensus phylogenetic tree, these three identified Microascus species were completely resolved from each other as well as from the other species with high bootstrap support and posterior probability, confirming the correct morphological identification and the effectiveness of the used genomic region. To the best of our knowledge, these three Microascus species are new to the funga of Iran. Additionally, the Alhagi maurorum and Cannabis sativa plants are reported here as new hosts (matrix nova) for M. cinereus and M. trigonosporus, respectively, in the world.