فهرست نشریات علمی دسترسی آزاد ایران

Dry bubble disease, caused by Lecanicillium fungicola, is a major challenge in button mushroom cultivation worldwide. This study examined the genetic diversity of L. fungicola isolates collected from various regions of Alborz, Isfahan, and Khuzestan provinces. Ten ISSR primers were employed to assess genetic diversity among the isolates. A total of 224 amplified fragments were obtained, with a similarity coefficient of 0.42 to 0.82 among isolates. Based on a similarity coefficient of 67%, the isolates were categorized into six groups. The average Polymorphism Information Content (PIC) was 0.772, with the highest PIC observed at 0.888, indicating a polymorphism rate of 96.8%.  Furthermore, the efficacy of six commercial fungicides (including Trifloxystrobin, Copper Oxide, Iprodione + Carbendazim, Penconazole, Neem, and Carbendazim) as well as four plant essential oils (Thyme, Peppermint, Eucalyptus, and Rosemary) on mycelial growth of the isolates was assessed at various concentrations using the disc-diffusion method. The results revealed varying levels of sensitivity to fungicides, with the isolate exhibiting the highest growth rate also showing high sensitivity to the fungicides. Among the tested fungicides, Iprodione + Carbendazim exhibited the highest inhibition. Peppermint Essential oil at a concentration of 0.8 ppm had the highest mycelial growth inhibition. This study provides valuable insights into the genetic diversity of L. fungicola isolates, their various level of sensitivity to fungicides, and the potential use of essential oils in disease management. It offers a foundation for future research on resistance evolution and integrated disease control strategies.