Expansion of Human Marrow Derived Mesenchymal Stem Cells and their Transdifferentiation Potential
نویسندگان
1 Department of Hematology, Tarbiat Modares University, Tehran, Iran
2 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz
3 National Institute of Genetic Engineering and Biotechnology
4 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz
5 1 Stem Cell and Transgenic Technology Research Center, 2Transplant Research Center, Shiraz University of Medical Sciences, Shiraz
6 National Institute of Genetic Engineering and Biotechnology
7 National Institute of Genetic Engineering and Biotechnology
doi
چکیده
Background: The ability of mesenchymal stem cells (MSCs) to differentiate into other cell types makes these cells an attractive therapeutic tool for cell transplantation. In order to provide a source of human MSCs for autologus cell-based therapy, we have expanded MSCs from the bone marrow and analyzed the biological identities and transdifferentiation potential.Methods: The bone marrow of healthy donors was aspirated from the iliac crest. The adjacent cells expanded rapidly and maintained with periodic passages until a relatively homogeneous population was established. The identification of these cells was carried out by differentiation potential into the osteocytes and adipocytes. Transdifferentiation of human MSCs into hepatocyte-like cells was undertaken in response to a specific culture condition.Results: The differentiation of MSCs into osteoblast is determined by deposition of a mineralized extracellular matrix. Adipocytes are identified by their morphology and staining. Hepatic cells were demonstrated in vitro functions characteristic of liver cells.Conclusion: We have defined conditions under which human MSCs can be isolated and expanded from humanbone marrow. These cells can be amplified about 108-fold in 6 weeks, and are capable of transdifferentiation intothe cells of another developmental lineage.
