In Vitro Assessment of Anti-Streptococcus Mutans Potential of Honey
نویسندگان
1 Department of Oral Medicine, Shiraz Dentistry School
2 Department of Oral Medicine, Shiraz Dentistry School
3 Shiraz Dentistry School, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Bacteriology and Virology, Shiraz Medical School
5 Shiraz Dentistry School, Shiraz University of Medical Sciences, Shiraz, Iran
doi
چکیده
Background: Honey is one of the traditional medicines used for treatment and prevention of various illnesses.This study aims to assess the in vitro antimicrobial effect of honey on Streptococcus mutans.Methods: This experimental study was conducted in Shiraz University of Medical Sciences. The Minimal Inhibitory Concentration/MIC effect of a type of commercial honey on S. mutans isolates was investigated by Agar well diffusion and broth microdilution assays. For comparison a carbohydrate solution with sugar content similar to that of natural honey was used as a control.Results: The MIC values of honey for 88.3% of tested strains were 75% v/v that were significantly different of the MIC values of the carbohydrate solution (p<0.001) and the average inhibition zone of undiluted honey on the S.mutans isolates was 13 mm. No inhibition zone was observed for undiluted carbohydrate solution in any isolates(p<0.001).Conclusion: Honey (Apis mellifera) has bacteriostatic activity when tested in vitro. However, pharmacological standardization and clinical evaluation of this effect are essential before using honey as a preventive measure for dental caries.
