The effect of 0.02%Mitomycin-C drop in prevention of epithelial ingrowth in corneal stroma of rabbit as an animal experimental model
نویسندگان
1 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
7 Poostchi Eye Research Center, Department of ophthalmology, Khalili Hospital, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
doi
چکیده
Background: Mitomycin–C (MM-C) is an alkylating anti-tumor antibiotic, which interrupts DNA replication andinhibits routine synthesis and cell mitosis. This study was undertaken to evaluate the effect of MM-C on theepithelial ingrowth which is a sight threatening complication after corneal surgeries.Methods: This study was conducted on 50 corneas of 25 Dutch Albino rabbits. Lamellar keratectomy was performedand after the application of MM-C 0.02 % or balanced salt solution (control group), the epithelial cells wereimplanted on the stromal bed. After sixty days, light microscopic exam was performed on the excised corneas.Results: Three eyes in the MM-C group and no eye in the control group showed epithelial cell growth under thecorneal flap.Conclusion: In this study, application of MM-C 0.02% drop at the time of operation had no preventive role in thecorneal epithelial cell proliferation under rabbit’s corneal flap. So, implantation of the epithelial cells under thecorneal flap is not the sole causative factor of epithelial ingrowth in the stroma of the rabbit’s cornea.
