فهرست نشریات علمی دسترسی آزاد ایران

The Gundelia tournefortii is considered one of the plants that harbor the most bioactive components. They can potentially be used to treat several illnesses, especially those involving the antioxidant system. Few studies have dealt with these plants in Iraq. Therefore, this study aims to determine, extract, and investigate the bioactivity of some plant extracts. The aerial parts of the plant were collected from Sulaimani governorate, Iraq. The plant's methanolic extract was obtained using Soxhlet. The phenolic acid primary screening was conducted using phytochemical tests, followed by secondary screening using Reverse-Phase HPLC-UV-Vis. Two specific phenolic acid types, rutin and gallic acid, were isolated and identified using preparative liquid chromatography and HPLC, respectively, along with FTIR spectroscopy. Then, the antioxidant and IC50 value of the methanolic extract was measured. Several phenolic compounds were detected in variable volumes, including rutin (32.9 ppm), kaempferol (90.1 ppm), apigenin (51.4 ppm), gallic acid (71.4 ppm), quercetin (74.1 ppm), myricetin (44.3 ppm), and catechin (78.9 ppm). A high free radical scavenging activity for methanol extract (IC50 value of 381.189µg/mL), compared to the control (vitamin C: 416.667µg/mL), was obtained. These findings indicate that the methanolic extract exhibits superior antioxidant activity to vitamin C and shows the significance of the antioxidant effectivity of G. tournefortii, which can be potentially used as an antioxidant supplement. It is essential to note that this study is the first to successfully isolate rutin and gallic acid from the aerial parts of the Iraqi G. tournefortii.