Scopolamine-induced memory impairment in mice: Soursop leaf extract and fractions protect the hippocampus and prefrontal cortex
نویسندگان
1 Neuropharmacology Unit, Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
2 Neuropharmacology Unit, Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
3 School of Nursing, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana
4 Department of Pharmacology & Therapeutics, Faculty of Basic Clinical Sciences, Bowen University, Iwo, Nigeria
5 Department of Pharmacology & Therapeutics, Faculty of Medicine and Pharmacy, Kampala International University in Tanzania, Dar Es Salaam, United Republic of Tanzania
6 Neuropharmacology Unit, Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
doi
10.22038/ijbms.2024.80289.17379چکیده
Objective(s): Annona muricata” (soursop) is a medicinal plant with diverse ornamental, consumptive, and pharmacological importance. This study was designed for its anti-amnesic potential in mice. Materials and Methods: The crude extract was fractionated with n-hexane, ethyl acetate, and aqueous methanol. The crude and fractions were tested in vitro for the anti-oxidant radical scavenging activity (DPPH), total flavonoid and phenolic content, and their acetylcholinesterase inhibitory activity. Thin-layer chromatography was used to determine the phytochemicals contained in the fractions and their purity. Neurobehavioral models like the Open Field Test, Novel Object Recognition Test, Y-Maze, and Morris Water Maze were used to evaluate the action of AMME (Annona muricata methanol extract) and AMAMF (Annona muricata aqueous methanol fraction). Results: The AMME and AMAMF significantly reduced the serum levels of myeloperoxidase and arginine (P<0.05). They also modulate the hippocampal and prefrontal acetylcholinesterase and glutamic acid decarboxylase activities (P<0.05). The 25 mg/kg AMAMF significantly affected short-term memory (P<0.05). The AMAMF and AMME significantly enhanced the prefrontal and hippocampal tissue levels of glutathione and superoxide dismutase. During the in vitro AchE assay on all fractions, the AMME and AMAMF consistently showed the greatest percentage of inhibitory activity. They inhibited 50% of the AchE enzymes with the lowest concentration. Conclusion: The study showed the neuroprotective effects of AMME and AMAMF in memory impairment models. The extracts showed potent AChE inhibition and positive effects on memory and anti-oxidant enzyme levels.
