Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.
نویسندگان
1 Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Plant Bio-Product group, Agricultural Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran. Iran
3 Plant Bio-Product group, Agricultural Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran. Iran
4 Department of Seed and Plant Improvement Research, Hamedan, Agricultural and Natural Resources, Research and Education Center, Agricultural Research, Education and Extension Organization, Hamedan, Iran
5 Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
doi
10.22092/jmpb.2021.354951.1369چکیده
The tissue culture of Nigella arvensis L. was carried out on an MS medium supplemented with various concentrations of auxins and cytokinins. Media fortified with different concentrations (0.0, 0.5, 1.0 and 1.5 mg/L) of 2, 4-D, NAA or IBA in combination with kinetin or BAP (1.5 mg/L and 2 mg/L) were used to induce and grow callus in present and absent of light. The experimental design was performed as a randomized block factorial design with three replications. Following callus culture, antioxidant enzymes activity and total phenolic contents were evaluated. The maximum callus induction (80.9%) was obtained with 1.0 mg/L 2, 4-D and 1.5 mg/L/ kinetin in the presence of light. Plant growth regulators combination and concentration, as well as photoperiod, affected the activity of antioxidant enzymes. The highest activities of catalase and peroxidase and total phenolic contents were measured in media with BAP combinations. On the contrary, the activity of superoxide dismutase was at the least in all BAP combinations. Overall, light intensified the activities of enzymes, but it had a negative effect on total phenolic contents.
