UPLC-QTOF-MS Profiling and In Silico Evaluation of Beta-Secretase Inhibitors from Ethyl Acetate Fraction of Paraboea leuserensis B.L. Burtt: Towards a Potential Therapeutic Approach for Alzheimer’s Disease
نویسندگان
1 Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
2 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia
3 Department of Pharmacology, Faculty of Pharmacy & Bio-Medical Sciences, MAHSA University, Selangor 42610, Malaysia
4 Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia
5 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
6 Department of Chemistry, Faculty of Science and Technology, Universitas Islam Negeri Sulthan Thaha Saifuddin, Jambi 36361, Indonesia
7 Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
doi
10.48309/jmcs.2026.558207.2900چکیده
This research is dedicated to investigating the bioactive compounds of the Paraboea leuserensis B.L. Burtt ethyl acetate fraction as potential beta-secretase 1 (BACE1) inhibitors, a central enzyme in amyloid beta (Aβ) peptide production that oligomerizes to create neurotoxic Alzheimer's disease plaques. Since BACE1 is a central enzyme in the amyloidogenic pathway, enzyme inhibition presents a promising therapeutic approach to delaying or even preventing the development Alzheimer's disease. Phytochemical analysis of the extract provides evidence of high phenolic acid and flavonoid content, two classes of secondary metabolites well known for their neuroprotective potential. In vitro assays illustrate that the extract significantly suppresses the activity of BACE1, a marker that renders the natural product pharmacologically active as a preventive agent for neurodegenerative disorders. To further improve the bioavailability and effectiveness of such bioactive molecules, an alternative nanophytotherapy strategy based on nanoparticle formulation has also been explored. The work here opens up new avenues to explore the use of Paraboea leuserensis as a source of a therapeutic candidate and forms the basis for future studies on identifying active constituents and determining molecular mechanisms of action.
