Bioactive Volatiles from Allium Sativum and Chrysanthemum Morifolium: GC-MS Analysis and Anticancer Activity Against Hepatic Cells
نویسندگان
1 Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Lebanese University, Beirut P.O. Box 6573, Lebanon
2 Department of Medical Laboratory Science, Faculty of Health Sciences, Al-Maaref University, Lebanon
3 College of Energy and Environmental Sciences, Department of Environment, A l-Karkh University of Sciences, Baghdad, Iraq
4 Doctoral School of Science and Technology, Research Platform for Environmental Science (PRASE), Lebanese University, Beirut P.O. Box 6573, Lebanon
5 Department of Chemistry, Faculty of Sciences, Islamic University of Lebanon, Khaldeh, Lebanon
6 Institut Européen des Membranes, Université de Montpellier, Montpellier, France
7 Department of Chemistry, Faculty of Sciences, Islamic University of Lebanon, Khaldeh, Lebanon
8 College of Engineering and Technology, American University of the Middle East, Egaila 54200, Kuwait
9 Department of Biological Sciences, Faculty of Sciences, Beirut Arab University, Tripoli 1300, Lebanon
doi
10.48309/jmcs.2026.536014.2846چکیده
Chrysanthemum morifolium and Allium sativum are significant medicinal plants with diverse biological activities attributed to their active chemical compounds, including volatile molecules. This study aimed to analyze the volatile compounds and evaluate the cytotoxic potential of hydromethanolic extracts from A. sativum and C. morifolium against liver cancer cells. Plant materials were extracted using hydromethanolic solvents and analyzed by GC-MS. Cytotoxic activity was evaluated using MTT assay against HepG2 hepatocellular carcinoma cells and WRL-68 normal liver cells at concentrations ranging from 12.5 to 400 µg/mL. GC-MS analysis revealed diverse volatile compounds in both extracts. A. sativum extract demonstrated superior cytotoxic activity with an IC₅₀ of 66.44 µg/mL, while C. morifolium showed an IC₅₀ of 100.9 µg/mL against HepG2 cells. At the highest concentration (400 µg/mL), A. sativum extract achieved 64.3% growth inhibition of HepG2 cells, while C. morifolium extract showed 39.6% inhibition. Both extracts demonstrated significantly lower toxicity toward normal WRL-68 cells. Both A. sativum and C. morifolium extracts exhibit promising anticancer potential against liver cancer cells with selective cytotoxicity, supporting their potential as natural therapeutic agents for liver cancer treatment.
