Intraperitoneal E.coli–Induced Sepsis: A Preliminary Study of Rat Model
نویسندگان
1 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
2 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
3 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
4 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
5 Pathology Anatomy, Faculty of Medicine, Universitas Muhammadiyah Surakarta, Indonesia
6 Faculty of Medicine, Universitas Muhammadiyah Surakarta, Indonesia
7 Internal Medicine Department, Faculty of Medicine, Universitas Muhammadiyah Surakarta, Indonesia
8 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
9 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
10 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
11 Internal Medicine Department, Faculty of Medicine, Universitas Sebelas Maret Surakarta, Indonesia
12 Public Health Department, Faculty of Medicine, Universitas Sebelas Maret, Indonesia
13 Faculty of Medicine, Universitas Muhammadiyah Surakarta, Indonesia
doi
10.26655/JMCHEMSCI.2025.1.9چکیده
Sepsis remains a complex clinical challenge, with experimental animal models being crucial for developing effective therapeutic strategies. Current approaches often struggle to establish clinically relevant animal models that accurately represent human sepsis pathogenesis. This study aimed to determine the optimal intraperitoneal Escherichia coli (E. coli) dose for inducing a reproducible sepsis state in male rats. A preliminary experimental study was conducted using male Rattus norvegicus rats divided into three groups: a control group and two sepsis groups induced with E. coli at doses of 1×106 and 1.5×106 CFU/kg. Biomarkers including neutrophils, procalcitonin (PCT), interleukin-6 (IL-6), and malondialdehyde (MDA) were measured at 24-, 48-, and 72-hours post-induction. Histopathological examinations of liver, kidney, spleen, and heart tissues were performed using hematoxylin and eosin staining. Both E. coli doses significantly increased inflammatory and infection biomarkers. Dose of 1.5×106 CFU/kg demonstrated higher biomarker levels and more severe organ damage compared to control group and 1×106 CFU/kg dose. In dose of 1.5×106 CFU/kg group, Neutrophil levels increased from baseline 1.4×103 to 7.71×103 mmol/dL, IL-6 from 20.72 to 77.49 pg/mL, MDA from 1.37 to 12.15 nmol/mL and PCT from a normal level of 3.1 to 5.79 ng/mL within 72 hours, meeting the criteria for sepsis. Histopathological examination revealed significant organ damage, with higher induction doses correlating with increased histopathological damage scores. Intraperitoneal E. coli induction at 1.5×106 CFU/kg effectively creates a reproducible sepsis model in rats, providing a valuable experimental approach for studying sepsis pathogenesis and potential interventions.
